Product: Anti-Dnase II (CT), Polyclonal
Cat. No: PC-031 (100 μg)
Background:
Apoptosis is characterized by several
morphological nuclear changes including
chromatin condensation and nuclear
fragmentation. These changes are triggered by
the activation of members of caspase family,
caspase activated DNase, and several novel
proteins including AIF and Acinus. DNase II
causes both chromatin condensation and DNA
fragmentation. The genes encoding human,
porcine, and murine DNase II have been cloned.
The DNase II gene encodes a 40 kDa
proenzyme. The mature enzyme consists of two
non-identical subunits, the 32 kDa (a) and 12
kDa (b) chains, generated by proteolytic
processing. Overexpression of DNase II induces
chromatin condensation. DNase II is
ubiquitously expressed in human tissues.
Specificity:
Antibody recognizes the approximately 32 kDa a
chain of human DNAse II. The antiobdy does not
recognize the 12 kDa b chain.
Positive control: Tissue lysate of human spleen.
An approximately 32 kDa (a chain) band should
be detected. The antiobdy doesn’t recognize the
12 kDa b chain.
Species Reactivity:
Human, other species not tested.
Ig Isotype:
Rabbit IgG
Immunogen:
A peptide corresponding to amino acids 347 to
360 of human DNase II precursor.
Format:
Immunoaffinity chromatography purified IgG,
100 μg in 200 μl of PBS containing 0.02%
sodium azide.
Storage and Stability:
Stable for at least one year when stored at 4°C.
Applications and Suggested Dilutions:

Western blot: analysis of DNase II in human
spleen tissue lysate in the absence (lane 1) or
presence (lane 2) of blocking peptide with
anti-DNase II (CT) at 1:500 dilution.
The optimal dilution for a specific application
should be determined by the researcher.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.