分子量:68.7 kDa

纯度:>98% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Human pIgR, His Tag by the LAL method.

Buffer:PBS, pH7.4

生物活性:Measured by its binding ability in a functional ELISA. Immobilized Human pIgR, His Tag at 2 μg/mL, the concentration of human IgM that produces 50% of the optimal binding response is appoximately 20200 ng/mL.

产品特性:Human pIgR, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 68.7 kDa. The predicted N-terminus is Lys 19. The reducing (R) protein migrates as 85-95 kDa in SDS-PAGE  due to glycosylation.

产品背景:Polymeric immunoglobulin receptor (PIGR) is also known as Poly-Ig receptor, which can be  cleaved into membrane secretory component (SC). PIGR is a type I transmembrane glycoprotein or a member of the immunoglobulin superfamily, and is synthesized by secretory epithelial cells and delivered to the basolateral plasma membrane where it can bind larger polymers of IgA (pIgA ) and pentameric IgM. The complex is then transported across the cell to be secreted at the apical surface. During this process a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment.