F2 monoclonal antibody (M01), clone 1B7
产品名称： F2 monoclonal antibody (M01), clone 1B7
英文名称： F2 monoclonal antibody (M01), clone 1B7
产品编号： H00002147-M01
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against a partial recombinant F2.
- Immunogen:
- F2 (AAH51332, 263 a.a. ~ 362 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- Sequence:
- RNPDGDEEGAWCYVAGKPGDFGYCDLNYCEEAVEEETGDGLDEDSDRAIEGRTATSEYQTFFNPRTFGSGEADCGLRPLFEKKSLEDKTERELLESYIDG
- Host:
- Mouse
- Reactivity:
- Human
- Isotype:
- IgG
- Storage Buffer:
- In 1x PBS, pH 7.2
- Storage Instruction:
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
- MSDS:
Download
- Applications
- Western Blot (Transfected lysate)
- Western Blot analysis of F2 expression in transfected 293T cell line by F2 monoclonal antibody (M01), clone 1B7.
Lane 1: F2 transfected lysate(70 KDa).
Lane 2: Non-transfected lysate. - Protocol Download
- Western Blot (Recombinant protein)
- Protocol Download
- Immunoprecipitation
- Immunoprecipitation of F2 transfected lysate using anti-F2 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with F2 MaxPab rabbit polyclonal antibody.
- Protocol Download
- Sandwich ELISA (Recombinant protein)
- Detection limit for recombinant GST tagged F2 is approximately 1ng/ml as a capture antibody.
- Protocol Download
- RNAi Knockdown (Antibody validated)
- Western blot analysis of F2 over-expressed 293 cell line, cotransfected with F2 Validated Chimera RNAi ( Cat # H00002147-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with F2 monoclonal antibody (M01), clone 1B7 (Cat # H00002147-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Protocol Download
- In situ Proximity Ligation Assay (Cell)
- Proximity Ligation Analysis of protein-protein interactions between FGA and F2. HeLa cells were stained with anti-FGA rabbit purified polyclonal 1:1200 and anti-F2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
- Entrez GeneID:
- 2147
- GeneBank Accession#:
- BC051332
- Protein Accession#:
- AAH51332
- Gene Name:
- F2
- Gene Alias:
- PT
- Gene Description:
- coagulation factor II (thrombin)
- Omim ID:
- 176930
- Gene Ontology:
- Hyperlink
- Gene Summary:
- Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Mutations in F2 leads to various forms of thrombosis and dysprothrombinemia. [provided by RefSeq
- Other Designations:
- coagulation factor II,prothrombin B-chain,serine protease
- Interactome
- Related Disease
- Abortion, Habitual
- Abortion, Spontaneous
- Abruptio Placentae
- Activated Protein C Resistance
- Acute Disease
- Albuminuria
- Alcoholism
- Alzheimer Disease
- Alzheimer disease
- Amyotrophic Lateral Sclerosis
- Amyotrophic lateral sclerosis
- Anemia, Sickle Cell
- Anemia, sickle cell
- Angina Pectoris
- Anoxia
- Antiphospholipid Syndrome
- Antithrombin III Deficiency
- Apnea
- Arterial Occlusive Diseases