Rabbit Anti-CXCR4/FITC Conjugated antibody

FITC标记的细胞表面趋化因子受体4抗体

英文名称Anti-CXCR4/FITC中文名称FITC标记的细胞表面趋化因子受体4抗体别    名C-X-C chemokine receptor type 4; CXC-R4; CXCR-4; Stromal cell-derived factor 1 receptor; SDF-1 receptor; Fusin; Leukocyte-derived seven transmembrane domain receptor; LESTR; CD184 antigen; CXCR4_HUMAN.  Journal
PMID
IF
Application
Scientific Reports (2015)
26250571
5.5780
FCM
Molecular Medicine Reports (2015)
25625935
1.4800
FCM规格价格100ul/2980元 购买        大包装/询价说 明 书100ul  研究领域肿瘤  免疫学  细胞膜受体  细胞类型标志物  抗体来源Rabbit克隆类型Polyclonal交叉反应Human, Mouse, Rat, Cow, Rabbit, 产品应用Flow-Cyt=1:50-200 IF=1:50-200  
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.分 子 量40kDa细胞定位细胞膜 性    状Lyophilized or Liquid浓    度1mg/ml免 疫 原KLH conjugated synthetic peptide derived from the middle of human CD184 (211-254aa)亚    型IgG纯化方法affinity purified by Protein A储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.产品介绍background:
This gene encodes a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions and is located on the cell surface. It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells. Mutations in this gene have been associated with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. Monomer. Can form dimers.

Function:
Receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. Acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. Involved in hematopoiesis and in cardiac ventricular septum formation. Also plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 X4 isolates and as a primary receptor for some HIV-2 isolates. Promotes Env-mediated fusion of the virus.

Subunit:
Monomer. Can form dimmers.

Subcellular Location:
Cell membrane; Multi-pass membrane protein.

Tissue Specificity:
Expressed in numerous tissues, such as peripheral blood leukocytes, spleen, thymus, spinal cord, heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, cerebellum, cerebral cortex and medulla (in microglia as well as in astrocytes), brain microvascular, coronary artery and umbilical cord endothelial cells. Isoform 1 is predominant in all tissues tested.

Post-translational modifications:
Phosphorylated on agonist stimulation. Rapidly phosphorylated on serine and threonine residues in the C-terminal. Phosphorylation at Ser-324 and Ser-325 leads to recruitment of ITCH, ubiquitination and protein degradation.
Ubiquitinated by ITCH at the cell membrane on agonist stimulation. The ubiquitin-dependent mechanism, endosomal sorting complex required for transport (ESCRT), then targets CXCR4 for lysosomal degradation. This process is dependent also on prior Ser-/Thr-phosphorylation in the C-terminal of CXCR4. Also binding of ARRB1 to STAM negatively regulates CXCR4 sorting to lysosomes though modulating ubiquitination of SFR5S. Sulfation on Tyr-21 is required for efficient binding of CXCL12/SDF-1alpha and promotes its dimerization.
O- and N-glycosylated. Asn-11 is the principal site of N-glycosylation. There appears to be very little or no glycosylation on Asn-176. N-glycosylation masks coreceptor function in both X4 and R5 laboratory-adapted and primary HIV-1 strains through inhibiting interaction with their Env glycoproteins. The O-glycosylation chondroitin sulfate attachment does not affect interaction with CXCL12/SDF-1alpha nor its coreceptor activity. 

DISEASE:
Defects in CXCR4 are a cause of WHIM syndrome (WHIM) [MIM:193670]; also known as warts, hypogammaglobulinemia, infections and myelokathexis. WHIM syndrome is an immunodeficiency disease characterized by neutropenia, hypogammaglobulinemia and extensive human papillomavirus (HPV) infection. Despite the peripheral neutropenia, bone marrow aspirates from affected individuals contain abundant mature myeloid cells, a condition termed myelokathexis.

Similarity:
Belongs to the G-protein coupled receptor 1 family.

Database links:

Entrez Gene: 7852 Human

Entrez Gene: 12767 Mouse

Entrez Gene: 60628 Rat

Omim: 162643 Human

SwissProt: P61073 Human

SwissProt: P70658 Mouse

SwissProt: O08565 Rat

Unigene: 593413 Human

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

CXCR4是白细胞中的一种受体，在免疫系统中起着调整细胞运动的重要作用，目前多用于肿瘤细胞的生长、浸润性相关的研究。

该基因编码CXC趋化因子受体特异性基质细胞衍生因子-1。该蛋白具有7个跨膜区，位于细胞表面。它与CD4蛋白一起作用，以支持HIV进入细胞，并且在乳腺癌细胞中也高度表达。该基因的突变与突发性（疣、低丙种球蛋白血症、感染和骨髓囊肿）综合征有关。编码不同的异构体的交替转录剪接变异体已被表征。单体。