CD39抗体
Applications
| Tested Applications: |
ELISA, WB, IHC, FC, IP |
| Specificity Reaction: |
Human,Mouse,Rat; Other species are not tested.Please decide the specificity by homology |
| Positive WB detected in |
Jurkat cells, HL-60 cells, human liver tissue, mouse brain tissue, mouse liver tissue |
| Positive IP detected in |
Mouse liver tissue |
| Positive IHC detected in |
Human placenta tissue, human liver tissue, human tonsillitis tissue |
| Positive FC detected in |
Jurkat cells |
| Recommended dilution |
WB : 1:500-1:5000
IP : 1:200-1:2000
IHC : 1:20-1:200
FC : N/A |
CD39抗体Application key: WB=Western Blotting ,IHC=Immunohistochemistry, IF=Immunofluorescence
Images
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Jurkat cells were subjected to SDS PAGE followed by western blot with 19229-1-AP(ENTPD1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human placenta using 19229-1-AP(ENTPD1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human placenta using 19229-1-AP(ENTPD1 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-ENTPD1 (IP:19229-1-AP, 4ug; Detection:19229-1-AP 1:800) with mouse liver tissue lysate 4000ug.
1X10^6 Jurkat cells were stained with 0.5ug ENTPD1 antibody (19229-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
Product Information CD39抗体
| Source: |
Rabbit |
Purify: |
Antigen affinity purification |
| IsoType: |
IgG |
Storage: |
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. -20oC, Avoid freeze / thaw cycles. |
Immunogen Information
| Immunogen: |
CD39 fusion protein ag6165 |
Full Name: |
Ectonucleoside triphosphate diphosphohydrolase 1 |
Caculated Molecular
Weight: |
58 kDa |
Observed Molecular
Weight: |
75-95 kDa CD39抗体 |
| GenbankNo |
BC047664 |
GeneID: |
953 |
| Symbol |
ENTPD1 |
Synonyms |
ATPDase, CD39, DKFZp686D194, DKFZp686I093, Ecto apyrase, Ecto ATP diphosphohydrolase 1, Ecto ATPase 1, Ecto ATPDase 1, ENTPD1, FLJ40921, FLJ40959, NTPDase 1 |
Background
|
ENTPD1(Ectonucleoside triphosphate diphosphohydrolase 1) is also named as CD39, NTPDase 1, Ecto-ATPDase 1 and belongs to the GDA1/CD39 NTPase family. In the nervous system, it can hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission.It is a noncovalent tetrameric protein and that detergent inhibition of enzymatic activity is caused by dissociation of ectoapyrase tetramers into monomers(PMID: 9733785 ).And it is a 70- to 100-kDa protein that is heavily N-glycosylated(PMID:7930580). It has 3 isoforms produced by alternative splicing. CD39 preferentially occurred as a dimer that could be dissociated into monomeric forms. (PMID: 12694193). The soluble isoform of CD39(38-478 amino acid) locates to the extracellular part and 19229-1-AP recognizes both the 3 isoforms and soluble form of CD39 (38-478aa) molecule.
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