CYTOKERATIN 20抗体
Applications
| Tested Applications: |
ELISA, WB, IF, IHC |
| Cited applications |
IF |
| Specificity Reaction: |
Human,Mouse,Rat; Other species are not tested.Please decide the specificity by homology |
| Cited species |
Mouse |
| Positive WB detected in |
COLO 320 cells, HeLa cells |
| Positive IHC detected in |
Human colon tissue, human appendix tissue, human colon cancer tissue, human ovary tumor tissue, human stomach cancer tissue, rat bladder tissue, rat colon tissue |
| Positive IF detected in |
HeLa cells |
| Recommended dilution |
WB : 1:500-1:5000
IHC : 1:20-1:200
IF : 1:20-1:200 |
CYTOKERATIN 20抗体Application key: WB=Western Blotting ,IHC=Immunohistochemistry, IF=Immunofluorescence
Images
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COLO 320 cells were subjected to SDS PAGE followed by western blot with 17329-1-AP(KRT20 antibody) at dilution of 1:1500
Immunohistochemistry of paraffin-embedded human colon tissue slide using 17329-1-AP( KRT20 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human colon tissue slide using 17329-1-AP( KRT20 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HeLa cells using 17329-1-AP( KRT20 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Product Information
| Source: |
Rabbit |
Purify: |
Antigen affinity purification |
| IsoType: |
IgG |
Storage: |
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. -20oC, Avoid freeze / thaw cycles. |
Immunogen Information CYTOKERATIN 20抗体
| Immunogen: |
Cytokeratin 20 fusion protein ag11308 |
Full Name: |
Keratin 20 |
Caculated Molecular
Weight: |
424aa,48 kDa |
Observed Molecular
Weight: |
46 kDa |
| GenbankNo |
BC031559 |
GeneID: |
54474 |
| Symbol |
KRT20 |
Synonyms |
CK 20, CK20, Cytokeratin 20, K20, keratin 20, KRT20, KRT21, Protein IT |
Background
|
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9–K23, and the hair keratins Ha1–Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1–K8, and the hair keratins, Hb1–Hb6. Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa. CYTOKERATIN 20抗体
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