This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific for Neuropeptide Y (NPY) has  been  pre-coated  onto  a  microplate.  A  competitive  inhibition  reaction  is  launched  between Horseradish Peroxidase (HRP) labeled Neuropeptide Y (NPY) and unlabeled Neuropeptide Y (NPY) (Standards or samples) with the pre-coated antibody specific for Neuropeptide Y (NPY). After incubation the unbound conjugate is washed off. The amount of bound HRP conjugate is reverse proportional to the concentration of  Neuropeptide Y (NPY) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of  Neuropeptide Y (NPY) in the sample.