分子量:39 kDa

纯度:>95% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Human Galactowaldenase, His Tag by the LAL method.

Buffer:50 mM Tris,150 mM NaCl,pH8.0

生物活性:

产品特性:Human Galactowaldenase, His Tag is fused with a polyhistidine tag at the N-terminus, and has a calculated MW of 39 kDa. The predicted N-terminus is Met. The reducing (R) protein migrates as 39 kDa in SDS-PAGE .

产品背景:UDP-glucose 4-epimerase, a member of the NAD(P)-dependent epimerase (dehydratase) family, is also known as galactowaldenase (GALE), UDP-N-acetylgalactosamine 4-epimerase (UDP-GalNAc 4-epimerase) and UDP-N-acetylglucosamine 4-epimerase (UDP-GlcNAc 4-epimerase),which is a homodimeric epimerase found in bacterial, fungal, plant, and mammalian cells. GALE can catalyze two distinct but analogous reactions: the reversible epimerization of UDP-glucose to UDP-galactose and the reversible epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The reaction with UDP-Gal plays a critical role in the Leloir pathway of galactose catabolism in which galactose is converted to the glycolytic intermediate glucose 6-phosphate. It contributes to the catabolism of dietary galactose and enables the endogenous biosynthesis of both UDP-Gal and GALE (UDP-GalNAc) when exogenous sources are limited. Both UDP-sugar interconversions are important in the synthesis of glycoproteins and glycolipids.