SRF抗体-抗体-抗体-生物在线
上海信裕生物科技有限公司
SRF抗体

SRF抗体

商家询价

产品名称： SRF抗体

英文名称： SRFAntibody

产品编号： XY16821-1-

产品价格： null

产品产地： 中国/美国

品牌商标： XYbscience

更新时间： 2023-08-17T09:55:27

使用范围： WB,ELISA,IHC-P,IHC-F,IF

上海信裕生物科技有限公司
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 SRF抗体Applications

In-house applications ELISA, IHC, WB, IP, IF     Application not tested? Please see our Guarantee.
Published applications IHC Publications 1 citations –view here
Species specificity Human,Mouse,Rat; other species not tested.
Published Species Human
Images
Prev12NextView more validation data
  • 16821-1-AP;HeLa cellsHeLa cells were subjected to SDS PAGE followed by western blot with 16821-1-AP(SRF antibody) at dilution of 1:400
  • 16821-1-AP;human brainImmunohistochemical of paraffin-embedded human brain using 16821-1-AP(SRF antibody) at dilution of 1:50 (under 10x lens)
  • 16821-1-AP;human brainImmunohistochemical of paraffin-embedded human brain using 16821-1-AP(SRF antibody) at dilution of 1:50 (under 40x lens)
  • 16821-1-AP;HeLa cellsIP Result of anti-SRF (IP:16821-1-AP, 3ug; Detection:16821-1-AP 1:1000) with HeLa cells lysate 3000ug.
  • 16821-1-AP;HeLa cellsImmunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 16821-1-AP( SRF Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Positive WB detected in HeLa cells, HEK-293 cells, K-562 cells, MCF-7 cells, NIH/3T3 cells
Positive IP detected in HeLa cells
Positive IHC detected in Human brain tissue
Positive IF detected in HeLa cells
Recommended dilution WB : 1:200-1:1000
IP : 1:500-1:5000
IHC : 1:20-1:200
IF : 1:50-1:500
 SRF抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
Source Rabbit Purification method Antigen affinity purification
Isotype IgG Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC.
Immunogen Information SRF抗体
Immunogen SRF fusion protein ag10386 Full name Serum response factor (c-fos serum response element-binding transcription factor)
Calculated molecular
weight		 508aa,52 kDa Observed molecular 
weight		 65-70 kDa,40 kDa
GenBank accession 
number		 BC048211 Gene ID (NCBI) 6722
Gene symbol SRF Synonyms MCM1, Serum response factor, SRF
Background

Serum response factor (SRF) is a transcription factor that binds the serum response element (SRE), a sequence that mediates the transient response of many cellular genes to growth stimulation. SRF is required for cardiac differentiation and maturation due to the role in cardiac cell growth and muscle gene regulation. The calculated molecular weight of SRF is 51 kDa, but the phosphorylated SRF protein is 60-70 kDa. SRF抗体

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