分子量:18.2 kDa

纯度:>97% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Human IL-33, Tag Free by the LAL method.

Buffer:PBS, pH7.4

生物活性:Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells costimulated with anti-CD3.  The ED50 for this effect is typically 0.05-0.26 ng/mL.

产品特性:Human IL-33, Tag Free contains no "tag", and has a calculated MW of 18.2 kDa. The predicted N-terminus is Met. The reducing (R) protein migrates as 18-20 kDa in SDS-PAGE .

产品背景:Interleukin 33 (IL33) is known as C9orf26, DKFZp586H0523, DVS27, NF-HEV, NFEHEV, RP11-575C20.2,and is a cytokine belonging to the IL-1 superfamily. IL-33 induces helper T cells, mast cells, eosinophils and basophils to produce type 2 cytokines. IL-33 mediates its biological effects by interacting with the receptors ST2 (aka IL1RL1) and IL-1 Receptor Accessory Protein (IL1RAP), activating intracellular molecules in the NF-κB and MAP kinase signaling pathways that drive production of type 2 cytokines (e.g. IL-5 and IL-13) from polarized Th2 cells. In vivo, IL-33 induces the expression of IL-4, IL-5, and IL-13 and leads to severe pathological changes in mucosal organs.