蛋白酶是一类天然存在的调节酶，用于切割多肽链氨基酸之间的肽键。在蛋白表达和分离过程中，内源性细胞蛋白酶可以快速消化细胞蛋白，致使蛋白质样品质量和数量大幅减少，这是蛋白表征和分析所需的。BioVision引入了一个新的常规产品线，也是特别的蛋白酶抑制剂和混合物，可以防止体外蛋白水解。这些蛋白酶抑制剂和混合物简单易用，可用于抑制广泛的蛋白酶。抑制剂混合物套装包含最佳浓度的抑制剂的不同组合，为研究者提供多种选择，包括不含EDTA的形式。
蛋白质磷酸化是可逆的蛋白翻译后修饰（PTM）中最常见和最重要的形式，所有蛋白质在任何给定的时间高达30%可被磷酸化。大范围的生物过程受磷酸化与去磷酸化蛋白的控制。为了了解蛋白质的精确磷酸化状态，从组织和细胞裂解液中提取的磷酸化蛋白保持蛋白质的磷酸化状态是非常重要的。BioVision有五种不同的EZBlock™磷酸酶抑制剂混合物和两种新的通用的蛋白酶和磷酸酶抑制剂混合物，抑制蛋白质提取和纯化过程中蛋白酶和磷酸酶活性。
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Product Name
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Cat #
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Target Proteases/Phosphatases
|
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EZBlock™ Protease Inhibitor Cocktail
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K271-500
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Aspartic, Cysteine, Serine proteases,
and Esterases
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EZBlock™ Protease Inhibitor Cocktail,
EDTA-Free |
K272-1, 5, set
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Aspartic, Cysteine, Serine, Trypsin-like
proteases, Aminopeptidases, and Esterases
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EZBlock™ Protease Inhibitor Cocktail II
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K277-set
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Cysteine, Serine, Trypsin-like proteases, Aminopeptidases, Metalloproteases
and Esterases
|
|
EZBlock™ Free Protease Inhibitor
Cocktail II, Animal-Free |
K394-1,set
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Cysteine, Serine, Trypsin-like proteases, Aminopeptidases, Metalloproteases and
Esterases
|
|
EZBlock™ Protease Inhibitor Cocktail III
|
K278-set
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Aspartic, Cysteine, Serine, Trypsin-like
proteases, Aminopeptidases,
Metalloproteases |
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EZBlock™ Protease Inhibitor Cocktail IV
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K279-1,set
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Aspartic, Cysteine, Serine proteases,
and Metalloproteases
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EZBlock™ Free Protease Inhibitor
Cocktail V, EDTA-Free |
K290-1,set
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Cysteine and Serine proteases
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EZBlock™ Use Protease Inhibitor
Cocktail VI, General Use |
K291-1,set
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Cysteine, Serine proteases, Aminopeptidases
and Metalloproteases
|
|
EZBlock™ Protease Inhibitor Cocktail VII
|
K292-1,set
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Aspartic, Cysteine , Serine proteases,
Aminopeptidases and Metalloproteases
|
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EZBlock™ Protease Inhibitor Cocktail VIII
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K293-1,set
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Aspartic, Cysteine , Serine proteases,
Aminopeptidases and thermolysin-like
proteases
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EZBlock™ Free Protease Inhibitor
Cocktail VIII, DMSO-Free |
K395-1,set
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Aspartic, Cysteine , Serine proteases,
Aminopeptidases and thermolysin-like
proteases
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EZBlock™ Protease Inhibitor
Cocktail IX |
K294-1,set
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Cysteine proteases, including Calpains,
Cathepsins and Papain.
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EZBlock™ Free Protease Inhibitor Cocktail X, EDTA-Free
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K297-1,set
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Cysteine, Serine and Aminopeptidases
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EZBlock™ Free, Animal-Free Protease Inhibitor Cocktail XII, EDTA Free, Animal-Free
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K221-1,set
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Cysteine and Serine proteases
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EZBlock™ Free, Animal-Free Protease Inhibitor Cocktail XIII, EDTA Free, Animal-Free
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K222-1,set
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Aspartic , Cysteine and Serine proteases, Aminopeptidases, Acid proteases
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EZBlock™ Protease Inhibitor Cocktail XIV
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K223-1,set
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Cysteine proteases including Cathepsins
and Papain
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EZBlock™ Protease Inhibitor Cocktail XV
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K393-1,set
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Cysteine proteases including Cathepsins
and Papain
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EZBlock™ Phosphatase Inhibitor Cocktail I
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K273-1,set
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Acid phosphatases, Alkaline phosphatases,
Ser/Thr Phosphatases, Protein Tyrosine
Phosphatases
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EZBlock™ Phosphatase Inhibitor Cocktail II
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K275-1,set
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Acid phosphatases, Alkaline phosphatases,
Ser/Thr Phosphatases, Protein Tyrosine
Phosphatases
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EZBlock™ Phosphatase Inhibitor Cocktail III
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K276-1,set
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Alkaline phosphatases, Ser/Thr
Phosphatases (PP1 and PP2A)
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EZBlock™ Phosphatase Inhibitor Cocktail IV
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K282-1,set
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Acid phosphatases, Alkaline phosphatases,
Ser/Thr Phosphatases, Protein Tyrosine
Phosphatases
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EZBlock™ Phosphatase Inhibitor Cocktail XI
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K298-1,set
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Acid phosphatases, Alkaline phosphatases,
Protein Tyrosine Phosphatases
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EZBlock™ Universal protease &
Phosphatase Inhibitor Cocktail |
K283-1,set
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Cysteine, Serine, Trypsin-like proteases, Aminopeptidases, Metalloproteases and
Esterases, Acid phosphatases, Alkaline
phosphatases, Ser/Thr Phosphatases,
Protein Tyrosine Phosphatases
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EZBlock™ Universal protease &
Phosphatase Inhibitor Cocktail, EDTA-Free |
K284-1,set
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Cysteine, Serine, Trypsin-like proteases, Aminopeptidases, Metalloproteases and
Esterases, Acid phosphatases, Alkaline
phosphatases, Ser/Thr Phosphatases,
Protein Tyrosine Phosphatases
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